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Title   Ames test 에서 항암 항생제의 돌연변이유발 및 이에 미치는 Retinoic Acid , β - Carotene 및 Ascorbic Acid 의 영향 ( Effect of Retinoic Acid , β - Carotene , and Ascorbic Acid on the Mutagenicity of Some Anticancer Antiviotics )
Publicationinfo   1992 Jan; 024(04): 504-516.
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Abstract   Some anticancer antibiotics such as daunomycin, mitomycin C, and bleomycin are known to exert their anticancer effect by damaing host DNA through the generation of free radicals. In this study, effects of retinoic acid, B-carotene, ascorbic acid on the mutagenicity of the anti- cancer antibiotics were investigated by using preincubation Salmonella/microsome assay with Salmonella typhimurium TAl02 strain, which is particularly useful for the detection of oxidative mutagens. Appropriate concentrations af the antibiotics being incorporated into the preincubation mixture and minimal glucose agar were determined by checking MIC. using Microbiology System 2(MS-2, Abbott Co., U.S.A.). Alteration in the mutagenicity of the anti-cancer antibiotics was observed when equivolumes of 1 mM, 0.l mM, and 0.01 mM of retinoic acid and B-carotene, and 10 mM, 1 mM, and 0.1 mM of ascorbic acid was added to the preincubation mixture. The most strong mutagenicity was observed with mitomycin C, followed by daunomycin and bleomycin in order. Mutagenicity of daunomycin was significantly suppressed by retinoic acid(l mM, 0.1 mM, and 0.01 mM), B-carotene(1 mM and 0.1 mM), and ascorbic acid(10 mM and l mM). Mutagenicity of mitomycin C was suppressed only by 10 mM of ascorbic acid. Mutagenicity of bleomycin was suppressed only by 1 mM of retinoic acid. On the contrary, 0.l mM and 0.01 mM of both reitnoic acid and B-carotene, and 0.1 mM of ascorbic acid potentiated the mutagenicity of bleomycin significantly.
저 자   이준행(Joon Haeng Rhee),이나영(Nah Young Lee),정선식(Sun Sik Chung),이현철(Hyun Hul Lee)